Figure 4

miRNA quantification from different collection routes and biofluid volumes. Whole blood was collected from the tail vein (TV) of a C57 (wild-type) mouse and an mdx (dystrophic) mouse. The mice were sacrificed and blood collected from the jugular vein (JV) from the same animals. Serum was prepared and RNA extracted. (a) Relative miRNA abundance was determined for each sample by small RNA TaqMan RT-qPCR for miR-1, miR-133a and miR-206 (b) Fold changes for mdx vs C57 for tail vein and jugular vein collections. (c) RNA was extracted from 10 μl, 25 μl, 50 μl and 100 μl of serum and relative miRNA abundance determined by small RNA TaqMan RT-qPCR for miR-1, miR-133a and miR-206.