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Figure 3 | Biological Procedures Online

Figure 3

From: Isolation of T cells from mouse oral tissues

Figure 3

Specialized T cell subsets in MOIL isolated from the oral tissues of C57BL/6 mice. (A) Flow cytometric contour plots showing the expression of various immune cell markers in MOIL cells CD3+ CD4+ RORγt + cells are shown as Th17 cells, CD3 + CD4 + CD25 + Foxp3+ cells are shown as Tregs, CD11b- NK1.1- CD3- CD90.2+ CD45.2+ RORγt are shown as innate lymphoid cells (ILC). Percentages of marker expressing cells are shown in the gates. These data are representative of two independent experiments using cells pooled from 10 mice. (B) Percentage of CD4 + Foxp3 (top) and CD4+ RORγt (bottom), in MOIL as assessed by flow cytometry from 5 independent experiments (as in Figure 2A). Spleen (SPLN), cervical lymph nodes (CLN), mesenteric lymph nodes (MLN) and MGIL are used as controls. (C) Real time quantitative PCR analyses of mRNA levels of Foxp3, RORγt and GAPDH (house keeping gene control) in cells isolated from SPLN, CLN and MOIL, with SPLN normalized to “1” to calculate the relative mRNA levels. These data are representative of three independent experiments using 15 mice. P values were calculated by Ordinary one-way ANOVA test, with multiple comparisons, using Prism 6.0 (GraphPad Software, Inc.).

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