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Figure 2 | Biological Procedures Online

Figure 2

From: Isolation of T cells from mouse oral tissues

Figure 2

Leukocyte and CD4 cell enrichment in MOIL isolated from the oral tissues using our method. (A) Flow cytometric dot plots showing leukocyte gates (top), CD4 expression (middle) and CD25 and Foxp3 expression (bottom) in MOIL isolated by different methods (pooled from 10 mice) from different strains of mice (gated as shown). Mouse gut intra-epithelial and lamina propria leukocytes (MGIL) are shown as a control. (B) Flow cytometric dot plots showing leukocyte gates (top) and CD4 expression (bottom) before (left) and after (right) the percoll centrifugation (C) Contour plots showing CD45.2, CD3 and CD8 expression, which are the hematopoietic, T lymphocytic, and CD8+ T lymphocytic markers, respectively. Percentages of marker expressing cells are shown in the gates. All these data represent at least three to five independent experiments using at least 10- 15 mice.

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