Figure 4

Application of the anti–Rae-1 mAb in Western blotting and flow cytometry. A. Detection of Rae-1 recombinant protein in Western blotting using the 52A mAb. Rae-1b recombinant protein (20 μg and 5 μg) and control human IgG-Fc (20 μg) were loaded onto 10% sodium dodecyl sulfate–polyacrylamide gel. In the Western blot assay, the primary antibody was the 52A anti–Rae-1 mAb, and the secondary antibody was HRP goat anti-mouse IgG. B. Detection of Rae-1 expression levels in multiple murine cancer cell lines using the 52A anti–Rae-1 mAb. Murine tumor cells were not stained, stained with isotype control, or stained with the 52A anti–Rae-1 mAb. Rae-1 expression levels were assessed using flow cytometry. a, K7M3 cells; b, LLC cells; c, TC1 cells; d, CT26 cells; e, B16F10 cells.