Simultaneous splicing of multiple DNA fragments in one PCR reaction

Table 1 Comparison between classical overlap extension PCR (OE-PCR) and our simultaneous splicing overlap extension PCR (SSOE-PCR)

Method	Overlap extension phase			Fused DNA amplifying phase			Number of reaction system	Thermo cycling programs
	Outermost primers	Annealing temperature	Function of outermost primers	Outermost primers	Annealing temperature	Function of outermost primers	Number of reaction system	Thermo cycling programs
Classical OE-PCR	No need	Lower	_	Need	Higher	Yes	One by one	Separate
SSOE-PCR	Need	Higher	No	Need	Lower	Yes	One	Continuous

ISSN: 1480-9222