Figure 5

Detecting the digestion product of fusion DNAs by Eco RI in 1% agarose gel. (A) Lane 1: five fusion (D3-D7, 2550 bp) from lane 2 of Figure 2C; Lane 2: five fusion (D3-D7, 2550 bp) from lane 3 of Figure 4C; Lane 3: four fusion (D4-D7, 1550 bp) from lane 1 of Figure 2B; Lane 4: four fusion (D4-D7, 1550 bp) from lane 2 of Figure 4B; Lane 5: DNA molecular weight markers. Lines more than 1 kb indicated that the fused DNAs were not fully digested. (B) The PCR products were digested by Eco RI after purifying the target DNA (4050 bp in lane 1 of Figure 4D and 6050 bp in lane 2 of Figure 4E) by gel extraction kit and then amplifying them by PCR. Lane 1: six fusion (D2-D7); Lane 2: seven fusion (D1-D7); Lane 3: DNA molecular weight markers.