Figure 4
From: Simultaneous splicing of multiple DNA fragments in one PCR reaction
Electrophoretic analysis of different number of DNA fragments fusions in 1% agarose gel. (A) Two and three DNA fragments fusion: lane 1 (800 bp), D5:D6:D7 fusion; lane 2, DNA molecular weight marker; lane 3 (3250 bp), D2:D3:D4 fusion; lane 4 (3500 bp), D1:D2 fusion. (B) Four DNA fragments fusion (D4-D7): lane 1 (1550 bp), unpurified PCR products as templates; lane 2 (1550 bp), purified PCR products as templates; lane 3, DNA molecular weight marker. (C) Five DNA fragments fusion (D3-D7): lane 1, DNA molecular weight marker; lane 2 (2550 bp), unpurified PCR products as templates; lane 3 (2550 bp), purified PCR products as templates. (D) Six DNA fragments fusion (D2-D7): lane 1 (4050 bp, arrow), unpurified PCR products as templates; lane 2(4050 bp, arrow), purified PCR products as templates; lane 3, DNA molecular weight marker. (E) Seven DNA fragments fusion (D1-D7): lane 1, DNA molecular weight marker; lane 2 (6050 bp), unpurified PCR products as templates; lane 3(6050 bp), purified PCR products as templates.