Figure 1
From: Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods
UV spectrophotometric quantification of DNA by NanoDrop. (A) Quantification of undiluted samples showed that the DNA quantity in the samples with the length of fragments approx. 1500 bp and 500 bp significantly decreased compared to concentration of unfragmented samples. There was no difference between the concentration of unfragmented DNA samples and specimens with the fragment length of 150 bp. (B) The DNA concentration of 10-fold diluted samples was not affected by the level of fragmentation. ns, statistically non-significant; ***, p< 0.0001.