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Table 9 Thermocycle conditions for assembly of multimers into vector

From: A do-it-yourself protocol for simple transcription activator-like effector assembly

Cycle Number Temperature 1 Temperature 2 Temperature 3
1 37°C, 5 min   
2-20 20°C, 5 min 37°C, 5 min  
21    80°C, 20 min
Hold at 4°C