Table 9 Thermocycle conditions for assembly of multimers into vector
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Temperature 1 | Temperature 2 | Temperature 3 |
|---|---|---|---|
1 | 37°C, 5 min |  |  |
2-20 | 20°C, 5 min | 37°C, 5 min |  |
21 |  |  | 80°C, 20 min |
Hold at 4°C |  |  |  |