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Table 8 Components for assembly of multimer into vector

From: A do-it-yourself protocol for simple transcription activator-like effector assembly

Component Amount per 1 reaction (μL) Negative Control (μL)
Backbone Vector 1 1
3 Purified Multimers 5* 0
ATP 1 1
T7 Ligase 0.25 0.25
Combo Buffer II 1 1
BSa I enzyme 0.75 0.75
BSA 1 1
DNA-grade H2O 0 5
Total 10 10
  1. *The proportion of each multimer within these 5 μL can be adjusted to achieve a similar concentration of each multimer in the mix.