Table 8 Components for assembly of multimer into vector
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Component | Amount per 1 reaction (μL) | Negative Control (μL) |
|---|---|---|
Backbone Vector | 1 | 1 |
3 Purified Multimers | 5* | 0 |
ATP | 1 | 1 |
T7 Ligase | 0.25 | 0.25 |
Combo Buffer II | 1 | 1 |
BSa I enzyme | 0.75 | 0.75 |
BSA | 1 | 1 |
DNA-grade H2O | 0 | 5 |
Total | 10 | 10 |