Table 6 Components for multimer amplification
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Component | Amount per 1 reaction (μL) |
|---|---|
PCR buffer | 10 |
DNA-grade Water | 35.5 |
Multimer primer mix | 2.5 |
dNTP | 0.5 |
High-fidelity DNA polymerase | 0.5 |
total | 49 |