Table 2 Components for multimer assembly
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Component | Amount per 1 reaction (μL) |
|---|---|
Combo Buffer | 1 |
DTT | 1 |
ATP | 1 |
Bsm BI restriction enzyme | 0.75 |
T7 Ligase | 0.25 |
total | 4 |
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Component | Amount per 1 reaction (μL) |
|---|---|
Combo Buffer | 1 |
DTT | 1 |
ATP | 1 |
Bsm BI restriction enzyme | 0.75 |
T7 Ligase | 0.25 |
total | 4 |