Table 2 Components for multimer assembly
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
| Component | Amount per 1 reaction (μL) |
|---|---|
| Combo Buffer | 1 |
| DTT | 1 |
| ATP | 1 |
| Bsm BI restriction enzyme | 0.75 |
| T7 Ligase | 0.25 |
| total | 4 |
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
| Component | Amount per 1 reaction (μL) |
|---|---|
| Combo Buffer | 1 |
| DTT | 1 |
| ATP | 1 |
| Bsm BI restriction enzyme | 0.75 |
| T7 Ligase | 0.25 |
| total | 4 |