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Table 11 Thermocycle conditions for colony PCR

From: A do-it-yourself protocol for simple transcription activator-like effector assembly

Cycle Number Denature Anneal Extend
1 94°C, 3 min.   
2-31 94°C, 30 s 60°C, 30s 72°C, 2 min
32    72°C, 5 min
Hold at 4°C