Table 11 Thermocycle conditions for colony PCR
From: A do-it-yourself protocol for simple transcription activator-like effector assembly
Cycle Number | Denature | Anneal | Extend |
|---|---|---|---|
1 | 94°C, 3 min. |  |  |
2-31 | 94°C, 30 s | 60°C, 30s | 72°C, 2 min |
32 |  |  | 72°C, 5 min |
Hold at 4°C |  |  |  |