Figure 4
From: Expression, purification and molecular analysis of the human ZNF706 protein
Polyacrylamide gel SDS-PAGE 18% shows purification of the recombinant human ZNF706 protein by nickel-affinity chromatography. Lane M: molecular weight marker (PageRuler™ Unstained Protein Ladder - Fisher Scientific, Pittsburgh PA, USA); Lane 1: crude extract. Lane 2: insoluble fraction. Lane 3: soluble fraction. Lane 4: Column washed with buffer plus imidazole 10 mM. Lane 5. Column washed with buffer plus imidazole 20 mM. Lanes 6-7. ZNF706 protein elution with buffer plus imidazole 250 mM. Lane 8: ZNF706 protein elution with buffer plus imidazole 500 mM.