Figure 4From: A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectorsReal-time monitoring and quantification of TALE in living cells. Images of TALE-induced GFP reporter expression in HEK293 cells were taken at indicated time-points after co-transfection of Sox2-TALE-TF and its dual reporter. (b) Fold induction of luciferase was calculated as relative activity at each time-point compared to its corresponding control (dual reporter plus empty TALE-TF vector). Levels of luciferase activities are expressed as mean ± SD, n = 3.Back to article page