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Figure 2 | Biological Procedures Online

Figure 2

From: A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors

Figure 2

Specific activation of dual reporters by TALEs. (a) Upper two panels: TALE-TF designed to activate the Sox2 gene were placed under the control of either CMV or EF1-a promoter. The target sites were selected from the 200-bp proximal promoter region of human Sox2 as indicated by Sox2-TALE binding sites (SBS). Lower two panels: the corresponding customized dual reporter was constructed by inserting 3xSBS. A control reporter harboring 3xUBS was set as a negative control. (b) Upper panel: Images of TALE-induced GFP reporter expression in trasnfected HEK293 cells. Lower panel: Levels of luciferase activities in transfected HEK293 cells, as determined by quantitative luciferase assay, in comparison to mock controls. Mock control cells received the same amount of plasmid DNA, but the Sox2 TALE-TF plasmid was replaced by empty vector. Data are expressed as mean ± SD, n = 3.

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