Figure 1

First two steps in the selection of electroporation formulation for human adipose-derived stem cells (ASCs). Error bars expressed as mean × SEM (n = 3). *, indicating a significant difference was detected at p < 0.05. (a) Step 1 of buffer optimization. 1 × 10E6 cells were used for each cell transfection in 100 μL indicated buffers and different programs with use of Amaxa nucleofector. (b) Step 2 of buffer optimization. The key for polymer abbreviations is in the Methods section. Differentiation of each polymer was tested. None, optimum buffer without addition of any polymer.