Figure 7
From: Novel mouse mammary cell lines for in vivo bioluminescence imaging (BLI) of bone metastasis
4T1.2 luc3 cells are osteolytic in vivo. (A) 1 × 104 4 T1.2 luc3 (left) or 4 T1 luc2 cells (right) were injected into the proximal end of the left tibia of a 6-week old female Balb/c mice and visualized after i.p. injection of 150 mg/kg D-Luciferin using an IVIS Spectrum BLI system (day 18 after tumor cell injection). Whole body ventral view shows luciferase activity from tumor cells in the left tibia, validated with ex-vivo imaging of the tibia. (B) micro-CT images of cross sectional slices (4000 × 4000 pixels) (i) at the proximal and (ii) distal ends of the tibia reveal extensive osteolytic bone degradation (osteolytic damage highlighted with white arrows) near the injection site. Micro-CT images scanned using a Microphotonics Skyscan 1172 CT at a 6μm resolution and reconstructed using ImageVis3D imaging software.