Figure 6
From: Novel mouse mammary cell lines for in vivo bioluminescence imaging (BLI) of bone metastasis
Quantification of BLI intensity from ex-vivo 66c14 luc2, 4 T1.2 luc3, and 4 T1 luc2 metastatic organs. Bioluminescence intensities were quantified ex vivo in (A) spine, (B) lung, and (C) liver of mice orthotopically injected with 66c14 luc2 (white bars), 4 T1.2 luc3 cells (black bars), or 4 T1 luc2 (grey bars), 32 days (4 T1.2 luc3 and 66c14 luc2) or 28 days (4 T1 luc2) or after tumor cell injection. Spines from mice injected with 4 T1.2 luc3 cells showed significantly higher BLI intensity as compared to both 66c14 luc2 and 4 T1 luc2 injected mice. Data expressed as photons/sec/cm2 (mean ± SEM; n = 3-4, *p < 0.05, unpaired t-test).