Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes

Table 1 Synthetic arrays generated from different types of repeats

Repeat unit	Size of unit	Size of array	Fold increase
Human alphoid DNA ^24-28
2 mer	0.34 kb	40 kb	x118
2 mer/mutant*	0.34 kb	60 kb	x176
4 mer	0.68 kb	70 kb	x103
5 mer	0.85 kb	140 kb	x165
6 mer	1.02 kb	35 kb	x35
tetO-CENP-B/2 mer**	0.34 kb	50 kb	x147
tetO-CENP-B/2 mer**	0.34 kb	50 kb	x147
Human gamma-satellite DNA ²⁹
1 mer	0.24 kb	24 kb	x100
Mouse satellite DNA ²⁹
Major, 3 mer	0.7 kb	55 kb	x79
Minor, 4 mer	0.5 kb	10 kb	x20

*Dimer from a chromosome 17 alphoid 16-mer higher order repeat containing a mutated CENP-B biding motif (CENP-B box).
**Novel artificial alphoid dimers composed of one monomer from a chromosome 17 alphoid 16-mer higher order repeat linked to a wholly synthetic alphoid monomer based on a published consensus sequence. The natural monomers contain either a wild type or mutated CENP-B box. In the synthetic monomer, this was replaced with a 42 bp tetracycline operator (tetO), the binding site for E. coli tetracycline repressor (tetR).

ISSN: 1480-9222