Media composition influences yeast one- and two-hybrid results

Table 2 The source of yeast nitrogen base also impacts yeast two-hybrid results.

	Yeast Nitrogen Base	pH adjustment	5 days observation		6 days observation
			*p53*	*LAM*	*p53*	*LAM*
1	Sigma	-	-	-	+	-
2	Clontech	-	+	-	+++	-
3	Difco	-	-	-	++	-

In this assay using control plasmids provided by Clontech, pB42AD-T-Antigen should bind pLexA-53, in which the p53 protein is fused to LexA, resulting in blue colonies [12]. Conversely, T-Antigen should not bind Lamin produced by pLexA-LAM [11].

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