Table 2 Representation of master mix preparation.
From: A quantitative PCR method for measuring absolute telomere length
Reagents | Volumes for one sample (ul) | Final concentration |
|---|---|---|
Power SYBR Green master mix (2x) | 10 | 1x |
Primer (telomere-fwd (2 μM)) | 1 | 0.1 μM |
Primer telomere-rev (2 μM) | 1 | 0.1 μM |
H20 | 4 | Â |
DNA (5 ng/μl DNA) | 4 | 20 ng total |