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Table 2 Representation of master mix preparation.

From: A quantitative PCR method for measuring absolute telomere length

Reagents Volumes for one sample (ul) Final concentration
Power SYBR Green master mix (2x) 10 1x
Primer (telomere-fwd (2 μM)) 1 0.1 μM
Primer telomere-rev (2 μM) 1 0.1 μM
H20 4  
DNA (5 ng/μl DNA) 4 20 ng total