Figure 1

Real-time PCR protocols for MGB- (left) and SYBR-assay (right). Protocols and run conditions that were used for MGB- and SYBR- assays are described in the upper part. Below are the oligo sequences and oligo concentrations used to prepare the NEO and Rpp30 assays. For the MGB-assay we prepared two (20×) mixes, one (*) for the target gene (NEO) and one (**) for the reference gene (Rpp30). For both assays, the final concentration in each reaction was 900 nM for oligos, 250 nM for probes (MGB-assay only). The MGB assay was prepared using TaqMan 2× Master Mix (Applied Biosystems): a standard run protocol of two hours was used. The SYBR-assay was performed with Fast SYBR Green 2× Master Mix (Applied Biosystems); the fast protocol reported took only 40 min.