Table 2 Primer sequences for EGFR and GAPDH transcripts used for real-time quantitative reverse transcriptase polymerase chain reaction
From: Influence of RT-qPCR Primer Position on EGFR Interference Efficacy in Lung Cancer Cells
Name | Forward | Exon | Sequence | Locationa | Reverseb | Exon | Sequence | Locationa | Length (bp) |
|---|---|---|---|---|---|---|---|---|---|
q1 | EGFR 370 F | 2 | GGCACTTTTGAAGATCATTTTCTC | c.370_393 | EGFR 514R | 3 | CTGTGTTGAGGGCAATGAG | c.514_532 | 163 |
q2 | EGFR 1089 F | 7 | CGAGGGCAAATACAGCTT | c.1089_1106 | EGFR 1263R | 9 | AAATTCACCAATACCTATT | c.1263_1281 | 193 |
q3 | EGFR 2034 F | 15 | GGCAGGAGTCATGGGAGAA | c.2034_2052 | EGFR 2168R | 17 | GCGATGGACGGGATCTTAG | c.2168_2186 | 153 |
GAPDH | GAPDH 240 F | 3 | TTGCCATCAATGACCCCTTCA | c.240_260 | GAPDH 395R | 5 | CGCCCCACTTGATTTTGGA | c.395_413 | 173 |
GAPDH 820 F | 8 | TGAACGGGAAGCTCACTGG | c.820_837 | GAPDH 1106R | 9 | TCCACCACCCTGTTGCTGTA | c.1106_1125 | 306 | |
GAPDH 1016 F | 8 | ACCCACTCCTCCACCTTTG | c.1016_1034 | GAPDH 1175R | 9 | CTCTTGTGCTCTTGCTGGG | c.1175_1193 | 177 |