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Table 2 Morphometric measurements for which aberrant crypts were evaluated

From: A Method for Serial Tissue Processing and Parallel Analysis of Aberrant Crypt Morphology, Mucin Depletion, and Beta-Catenin Staining in an Experimental Model of Colon Carcinogenesis

Measurement type Measurement name Description with units Average value(mean±SD)
Classification CRYPTCOUNT Total number of crypts per ACF 6.7 ± 5.4
Area ACF_AREA Area of each ACF in square microns (μm2 × 104) 2.5 ± 1.5
  ACF_EPIAREA ACF epithelial area (μm2 × 104) (ACFAREA–CSUMAREA) 2.3 ± 1.3
  C_SUMAREA Sum of crypt area (μm2 × 103) 2.2 ± 2.5
  C_AVGAREA Average crypt area in square microns (μm2 × 102) 3.4 ± 1.7
Diameter ACF_DIAAVG ACF average diameter in microns (μm × 102) 1.7 ± 0.5
  C_AVGMAXDIA Crypt maximum diameter in microns (μm × 10) 3.0 ± 1.0
  C_AVGSIZELEN Crypt average size length in microns (μm × 10) 2.9 ± 1.1
Perimeter ACF_PERIM ACF perimeter in microns (μm × 102) 5.6 ± 1.6
  C_AVGPERIM Average crypt perimeter in microns (μm × 10) 6.8 ± 2.7
Roundness ACF_ROUND ACF roundness (1 = round, higher numbers indicate polygonal shape) 1.1 ± 0.1
  C_AVGROUND Average crypt roundness (1 = round, high numbers indicate polygonal shape) 2.2 ± 0.6
  C_ROUNDCAT Crypt roundness category: 0–4 1.7 ± 0.7
Density ACF_DEN ACF density 0.5 ± 0.2
  ACF_IOD ACF integrated optical density (area × density) (μm2 × 104) 1.2 ± 0.9
HID-AB staining P_SULFOAREA Percent sulfomucin area (HID stain only) (%) 55 ± 32
  P_SIALOAREA Percent sialomucin area (AB stain only) (%) 16 ± 20
  P_UAREA Percent unstained area (lacks sulfo- and sialomucin HID-AB stain; high numbers, e.g., ≥85% probably indicate MDF) (%) 30 ± 26
  MDF Mucin-depleted foci (≥85% PUAREA)