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Figure 6 | Biological Procedures Online

Figure 6

From: A Method for Serial Tissue Processing and Parallel Analysis of Aberrant Crypt Morphology, Mucin Depletion, and Beta-Catenin Staining in an Experimental Model of Colon Carcinogenesis

Figure 6

a H&E stain showing distinct ACF from surrounding normal crypts (×100); bar = 100 μm. b ACF at higher magnification (×400) with arrows indicating cigar shaped dysplastic nuclei. HID staining (sulfomucin) is evident in the goblet cells of surrounding normal crypts (diamond), but is absent in particular ACF. AB staining (sialomucin) can be seen in the goblet cells (arrowheads) within the ACF. However, the dysplastic crypt shows absence of staining for both mucins; bar = 50 μm.

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