Table 1
ChIP assay buffers | |
|---|---|
1× RIPA Buffer (50 ml) | |
Final concentration: | Volume of stock buffers: |
10 mM Tris–HCl, pH 7.5 | 500 μl of 1 M |
1 mM EDTA | 100 μl of 0.5 M |
1% Triton X-100 | 5 ml of 10% |
0.1% SDS | 500 μl of 10% |
0.1% Nadeoxycholate | 500 μl of 10% |
100 mM NaCl | 1 ml of 5 M |
Water | 42.4 ml |
2× RIPA Buffer (50 ml) | |
20 mM Tris–HCl, pH 7.5 | 1 ml of 1 M |
2 mM EDTA | 200 μl of 0.5 M |
2% Triton X-100 | 10 ml of 10% |
0.1% SDS | 500 μl of 10% |
0.2% Nadeoxycholate | 1 ml of 10% |
200 mM NaCl | 2 ml of 5 M |
Water | 35.3 ml |
Lysis Buffer (10 ml) | |
50 mM Tris–HCl, pH 8.0 | 500 μl of 1 M |
10 mM EDTA | 200 μl of 0.5 M |
1% SDS | 1 ml of 10% |
Water | 8.3 ml |
TE Buffer (50 ml) | |
10 mM Tris–HCl, pH 8.0 | 500 μl of 1 M |
10 mM EDTA | 1 ml of 0.5 M |
Water | 48.5 ml |
Elution Buffer (50 ml) | |
20 mM Tris–HCl, pH 7.5 | 1 ml of 1 M |
5 mM EDTA | 500 μl of 0.5 M |
50 mM NaCl | 500 μl of 5 M |
Water | 48 ml |