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Table 1

From: A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip

ChIP assay buffers  
1× RIPA Buffer (50 ml)  
Final concentration: Volume of stock buffers:
10 mM Tris–HCl, pH 7.5 500 μl of 1 M
1 mM EDTA 100 μl of 0.5 M
1% Triton X-100 5 ml of 10%
0.1% SDS 500 μl of 10%
0.1% Nadeoxycholate 500 μl of 10%
100 mM NaCl 1 ml of 5 M
Water 42.4 ml
2× RIPA Buffer (50 ml)  
20 mM Tris–HCl, pH 7.5 1 ml of 1 M
2 mM EDTA 200 μl of 0.5 M
2% Triton X-100 10 ml of 10%
0.1% SDS 500 μl of 10%
0.2% Nadeoxycholate 1 ml of 10%
200 mM NaCl 2 ml of 5 M
Water 35.3 ml
Lysis Buffer (10 ml)  
50 mM Tris–HCl, pH 8.0 500 μl of 1 M
10 mM EDTA 200 μl of 0.5 M
1% SDS 1 ml of 10%
Water 8.3 ml
TE Buffer (50 ml)  
10 mM Tris–HCl, pH 8.0 500 μl of 1 M
10 mM EDTA 1 ml of 0.5 M
Water 48.5 ml
Elution Buffer (50 ml)  
20 mM Tris–HCl, pH 7.5 1 ml of 1 M
5 mM EDTA 500 μl of 0.5 M
50 mM NaCl 500 μl of 5 M
Water 48 ml
  1. Containing 1% SDS and 50 μg/ml proteinase K prior to use
  2. All buffers must be 0.2 uM filtered sterilized, and are based on [41]