Figure 4
From: A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors
MIA-TF detected the activity of transcription factors in cells after drug treatment. a After treatment with 25 μM hydroquinone for 16 h, the MIA-TF method was used to detect the changes of NF-κB expression in HeLa cells. b After treatment with 100 μM desferrioxamine (DFO) for 16 h, the MIA-TF method was also used to detect the changes of HIF-1 expression in HeLa cells. The results are expressed as mean ± SD. *p < 0.05, n = 6. NBS biotin-labeled dsDNA containing five times NF-κB-binding sites, HBS biotin-labeled dsDNA containing seven HIF-binding sites.