Figure 3
From: A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors
Sensitivity of MIA-TF detection. a The MIA-TF method was used to detect different contents of recombinant human NF-κB p50 transcription factors. b The MIA-TF method was used to detect NF-κB activity in different contents of nuclear extract. c The MIA-TF method was used to detect HIF-1 activity in different contents of nuclear extract. The results are expressed as mean ± SD. *p < 0.05 and, compared with the control group (column II), n = 6. NBS biotin-labeled dsDNA containing five times NF-κB-binding site, HBS biotin-labeled dsDNA containing seven times HIF-binding site.