Figure 2
From: A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors
The MIA-TF method was used to detect the activity of transcription factors. a The MIA-TF method was used to detect the changes in NF-κB expression in 500 ng of nuclear extract. b The MIA-TF method detected the changes of HIF-1 expression in 500 ng of nuclear extract. Relative total mean = EventsR1 × MeanR1/EventsR2 × MeanR2. The results are expressed as mean ± SD. *p < 0.05, n = 6. NBS biotin-labeled dsDNA containing five NF-κB binding site, cNBS non-biotin-labeled NBS, HBS biotin-labeled dsDNA containing seven HIF binding sites, cHBS non-biotin-labeled HBS, VP16 non-biotin-labeled dsDNA containing no HBS and NBS.