Skip to main content

Table 1 Competition assay of different drugs with N,N'-ethylene-bis(iodoacetamide) on MDA-MB-231 and their effects on the inhibition of the bisthioalkylation of Cys-239 and Cys-354 of β-tubulin by N,N'-ethylene-bis(iodoacetamide)

From: Quick and Simple Detection Technique to Assess the Binding of Antimicrotubule Agents to the Colchicine-Binding Site

Compound Cellular target (Binding site) Drug treatment (µM) Competition assay Inhibition of EBI binding to β-tubulin
Dimethylsulfoxide N/A 0.5% N/A
EBI Tubulin (C-BS) 100 N/A
Colchicine + EBI Tubulin (C-BS) 2.5 Yes
Combretastatin A-4 + EBI Tubulin (C-BS) 2.5 Yes
Podophyllotoxin + EBI Tubulin (C-BS) 20 Yes
2-Methoxyestradiol + EBI Tubulin (C-BS) 100 Yes
Paclitaxel + EBI β-Tubulin (T-BS) 5 Yes
Vinblastine + EBI β-Tubulin (vinca) 5 No
Vinblastine + EBI β-Tubulin (vinca) 100 No
Verapamil + EBI Voltage-dependent calcium channel 100 No
Daunorubicin + EBI Topoisomerase II 50 No
  1. N/A not applicable, C-BS colchicine-binding site, T-BS taxol-binding site