Figure 1
From: Highly Efficient Stable Expression of Indoleamine 2,3 Dioxygenase Gene in Primary Fibroblasts
Construction of a lentiviral-based vector for delivering the IDO gene. a Schematic diagram of the IDO–mCherry lentiviral construct. b Fluorescence microscopy analysis of IDO-expressing cells. Panels a and b as well as c and d represent bright-field and fluorescent images of the IDO–mCherry (red) expression in non-transduced and transduced 293T cells, respectively. c Lentiviral vector-mediated IDO protein expression in transduced 293T cells. The arrow on the upper band shows the IDO protein. The IDO-lentiviral vector lanes represent two separate viral preparations. The blot was reprobed with a β-actin antibody as a loading control. d Kynurenine assay. Functional IDO activity was evaluated by measuring the content of kynurenine in the conditioned media of non-transduced and IDO-lentiviral vector-transduced cells. The data shown are the mean and standard deviation of kynurenine in conditioned media of three separate experiments.