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Table 2 Quality control of 42 GeneChips® a

From: Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis

Item

MG

MA

Overall

Expected

Background

55 ± 4

49 ± 3

52 ± 2

<100

Noise

2.3 ± 0.2

2.1 ± 0.2

2.2 ± 0.1

<5

Percent present (%)

65 ± 0.6

65 ± 0.8

65 ± 0.5

>35

3'/5' dap

2.0 ± 0.7

1.7 ± 0.5

1.8 ± 0.4

<3

3'/5' lys

1.0 ± 0.2

2.3 ± 0.6

1.7 ± 0.3

<3

3'/5' phe

3.3 ± 0.9

2.1 ± 0.5

2.7 ± 0.5

<3

3'/5' trp

1.9 ± 0.6

0.7 ± 0.1

1.3 ± 0.3

<3

5' Signal value bioB

1,562 ± 43

1,585 ± 65

1573 ± 39

Increasing stepwise trend (bioB, bioC, bioD, and cre)

5' Signal value bioC

4,660 ± 125

4,554 ± 125

4,607 ± 88

 

5' Signal value bioD

8,458 ± 229

8,232 ± 242

8,345 ± 165

 

5' Signal value cre

25,205 ± 730

24,492 ± 662

24,848 ± 490

 
  1. aValues are means ± SEM