Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage

Table 3 Phenotypic changes of MSCs exposed to endothelial differentiating conditions

MSCs were labelled with the fluorescent dyes described in the legend of Table 1 and observed under fluorescence microscopy. MSCs were grown under standard basal condition (Day 0) and under fibronectin (F) or gelatin (G) culture condition for 7 days (Day 7). There were no substantial differences between MSCs cultured with these two different media (F = G)
Cell counting was performed by calculating the cell average number of 10 randomly selected high-power fields (×200) of 3 separated experiments
Fluorescence score: 3+ 50–90%, 2+ 20–50%, 1+ <20%, - no fluorescence

ISSN: 1480-9222