Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage

Table 2 Comparison between the proliferation potential of treated PBMCs and BMMCs

	PBMC/F	PBMC/G	BMMC/F	BMMC/G
% dishes with confluent cells	0	30	60	100
Confluence time		>2 wk	<2 wk	<2 wk
% increase in 1st passage cell-number		0	50	70
% increase in 2nd passage cell-number			20	50

The trypan blue exclusion staining was performed to quantify cells which were grown under fibronectin (F) or gelatin (G) culture condition. Re-seeding was carried out when cells reached confluence. Cell counting was performed by calculating the cell average number of 10 randomly selected high-power fields (×200) of 5 separate experiments. The percentage increase in the post-confluent cell number was calculated 1 week after re-seeding

ISSN: 1480-9222