| | PBMC/F | PBMC/G | BMMC/F = BMMC/G |
|---|
| |
d7
|
d14
|
d21
|
d7
|
d14
|
d21
|
d7
|
d14
|
d21
|
AcLDL | 4+ | 4+ | 4+ | 4+ | 3+ | 2+ | 4+ | 3+ | 1+ |
BS-I | 4+ | 4+ | 4+ | 4+ | 3+ | 3+ | 4+ | 4+ | 4+ |
VEGFR-2 | 3+ | 4+ | 4+ | 4+ | 3+ | 3+ | 4+ | 4+ | 4+ |
CD31 | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ |
Macrophage | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ |
CD90 | - | - | - | - | - | - | - | - | - |
- Cells were observed by use of an inverted fluorescence microscope after 7, 14, and 21 days under fibronectin (F) or gelatin (G) culture condition. Cell counting was performed by calculating the cell average number of 10 randomly selected high-power fields (×200) of 3 separate experiments
- AcLDL and BS-I were Dil and FITC conjugated, respectively. Immunofluorescence of VEGFR-2, CD31, macrophage antigen, and CD90 was detected by FITC-conjugated polyclonal secondary antibodies. The pattern of markers evaluated for BMMCs gave the same results irrespective of the culture condition
- Fluorescence scores: 4+ >90%, 3+ 50–90%, 2+ 20–50%, 1+ <20%, - no fluorescence
