Figure 4

In vitro tubule-like structure formation by untreated MSCs or MSCs cultured with endothelial differentiating media. Untreated MSCs were cultured alone (a) or with HUVECs (b,c). MSCs pre-treated with fibronectin or gelatin medium were also cultured alone (d and g, respectively) or with HUVECs (e,f and h,i, respectively). FITC-UEA-1-labeled HUVECs co-cultured with MSCs are shown in (c), (f) and (i). The micrographs are representative of triplicate experiments performed using MSCs on Matrigel.