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Figure 2 | Biological Procedures Online

Figure 2

From: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf

Figure 2

PCR screening test for peanut immature pod-specific genes. PCR templates: peanut leaf cDNA library (lanes 1, 3, 5, 7, 9, 11, 13, 15, 17) and peanut immature pod cDNA library (lanes 2, 4, 6, 8, 10, 12, 14, 16, 18). PCR products: control gene (~194 bp; lanes 1 and 2); candidate genes that are only expressed in peanut pod: cluster 7 (~173 bp; lanes 3 and 4), cluster 31 (~152 bp; lanes 5 and 6), cluster 33 (~233 bp; lanes 9 and 10), cluster 15 (~218 bp; lanes 11 and 12), cluster 11 (~170 bp; lanes 13 and 14), cluster 8 (~80 bp; lanes 15 and 16); candidate genes that are more highly expressed in peanut immature pod than leaf: cluster 23 (~152 bp; lanes 7 and 8), cluster 24 (~160 bp; lanes 17 and 18). M DNA marker.

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