A comparison of glucose-stimulated calcium (GSCa) response for islets cultured overnight in 5.5 mM glucose (5 G) and 11 mM glucose (11 G). a GSCa for islets cultured in 5.5 mM glucose (solid, mean of n = 8) and 11 mM glucose (dashed, mean of n = 9). Arrows indicate the three phases of GSCa; stimulation with 11 mM glucose is indicated by the horizontal bar. b Mean amplitude of the calcium response during each phase (phase 0, 1, and 2) for islets cultured in 5.5 mM glucose (solid, n = 37) or 11 mM glucose (stripes, n = 34); * indicates p < 0.05. Note that although a difference in latency is apparent in (a), this not a consistent or significant effect in the larger data set.