Figure 2
From: A Practical Guide to Rodent Islet Isolation and Assessment
Assessment of islet viability by fluorescence intensity of propidium iodide. a, b Untreated (healthy) islets shown in brightfield (a, top panel) display only trace amounts of PI fluorescence (a, bottom panel). Islets treated overnight with a combination of cytokines (100 pg/mL TNF-α, 50 pg/mL IL-1β, 100 pg/mL IFN-γ) shown in brightfield (b, top panel), in contrast, display substantial PI fluorescence (b, bottom panel).