Figure 4
From: Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers
Effect of Rottlerin on the energy charge ( EC ). Cells were treated with 5 and 20 μM Rottlerin (R5 and R20), and high-energy phosphates (ATP, ADP, and AMP) were determined by CZE, as described in "Materials and methods". a Representative electropherogram of perchloric acid extracts from MCF-7 cells. A Control sample, B 30-min incubation with 20 μM Rottlerin. AU absorbance units. b The EC, represented by the ATP + ADP + AMP/0.5(ATP + AMP) ratio, was calculated at the indicated time points and expressed as % of the control (100%). Values are the average of three separate experiments and are expressed as mean ± SD. *p < 0.05.