Figure 2
From: Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers
Effect of Rottlerin on cell number and viability assessed by direct cell counting and MTT assay, respectively. MCF-7 cells were treated with 5 and 20 μM Rottlerin (R5 and R20) before adding MTT. a Between 1 and 24 h later, cell number was determined, and b cell viability was measured as described in "Materials and methods". Results are expressed as % of the control (100%). Values are the average of three separate experiments in quadruplicate and are expressed as mean ± SD. *p < 0.05.