Figure 2

Repeated blood samples over time (time-course study). Initially, both groups had blood samples collected without anesthesia (time = -10 min), and no significant difference was observed. At time = 0, group 1 received intraperitoneal injection of Hypnom/Dormicum. Subsequently, hemoglobin was measured at different time points, and the anesthetized group (group 1) had hemoglobin levels significantly lower than the non-anesthetized mice (group 2). *P < 0.05, **P < 0.01, ***P < 0.001.