Figure 4

Inhibition of formation of contractile microfilaments by cytochalasin B (CB) leads to a rapid drop of cell index values. BPH-1 cells were pretreated with transforming growth factor-β1 (10 ng/ml) for 68 h and treated with CB (10 μg/ml) for another 3 h. a F-actin was visualized by phalloidin-fluorescein isothiocyanate conjugate after 3 h of CB and/or vehicle treatment. b CB induces a rapid decrease of the normalized cell index (data were normalized at the time of 68 h) acquired with the use of an xCELLigence real-time cell analyzer system. The arrow indicates the time interval of CB addition.