Figure 4
From: A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
Kinetic study of C-654. Effect of UDP-GlcNAc or peptide concentration on C-654 activity. Assays were performed under (a), varying UDP-GlcNAc concentration from 0.05 to 0.5 mM while holding peptide concentration at 0.5 mM; b varying peptide concentration from 0.05 to 0.5 mM and holding UDP-GlcNAc concentration at 0.5 mM, respectively. The inset figures show double-reciprocal plots for UDP-GlcNAc and peptide, respectively. The zymological parameters of C-654 were indicated at the bottom. The error bars indicate the range of standard deviation of the experimental data.