Figure 3From: A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay Standard assay of pyruvate and UDP. a Standard assay of pyruvate was performed according to the manufacturer's protocol. b Standard assay of UDP was performed in the presence of PEP (0.7 mM) and PK (2 U). Both pyruvate and UDP concentration was ranged from 0.02 to 0.2 mM. The error bars indicate the range of standard deviation of the experimental data.Back to article page