Figure 2

Ectopic expression of WNT1 in the mouse intestine. a Reverse transcriptase-polymerase chain reaction (RT-PCR) for intestinal expression of human WNT1, green fluorescent protein (GFP), and Actin in uninjected, DsRed-injected, and WNT1-injected mice. b Gene expression levels of GFP (green) and WNT1 (red) in isolated crypt and villus epithelium and mesenchymal cells by qRT-PCR. Levels normalized to internal reference gene and relative to crypt values. Samples from representative animal assayed in triplicate, data reported as mean ± std. c Immunoblot probed with antibodies to WNT1 and Actin from proximal, mid, and distal small intestine (PSI, MSI, and DSI) of WNT1- and DsRed-infected animals. d Tissue section from WNT1-injected mice stained with antibodies to GFP (green) and counterstained with Hoechst dye. Arrowheads indicate GFP-expressing mesenchymal cells. White box is enlarged in e. Dashed line represents epithelial-mesenchymal boundary; solid line represents apical epithelial border. Bar = 25 μm.