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Figure 1 | Biological Procedures Online

Figure 1

From: Lentiviral-Mediated Transgene Expression Can Potentiate Intestinal Mesenchymal-Epithelial Signaling

Figure 1

Lentiviral-mediated gene delivery to the mouse small intestine. a Schematic of virus production in 293T cells and mouse injection. Mice were injected at postnatal day 1 and sacrificed for analysis at later time points. b Green fluorescent protein (GFP) gene expression by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) in the gastrointestinal tract and other organs. GFP expression is normalized to glyceraldehyde phosphate dehydrogenase expression and presented relative to levels in the stomach. Graph displays triplicate samples from representative animals. Data displayed as mean ± std. c GFP expression in isolated intestinal crypt and villus epithelium and mesenchymal cells by qRT-PCR. Graph displays triplicate samples from representative animal. Data displayed as mean ± std. d Hematoxylin and eosin stained normal intestinal crypt-villus unit. Solid line indicates apical epithelial border; dashed line indicates epithelial-mesenchymal border. e–g Co-labeling of DsRed-injected intestine with antibodies to GFP (green) and DsRed (red). Yellow box in e and f is enlarged in g. Arrowheads indicate dual expressing mesenchymal cells. Dashed line indicates epithelial-mesenchymal border. Solid white line in g marks the apical border. Bar = 25 μm.

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