Control | Interferon-alpha treated | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
4 h | 24 h | 4 h | 24 h | |||||||||
a | ME-15 | HuH7 | CHF | ME-15 | HuH7 | CHF | ME-15 | HuH7 | CHF | ME-15 | HuH7 | CHF |
Validated microRNAs | ||||||||||||
hsa-miR-19a | 13,617 | 6,726 | -1.02 | 18,318 | 17,178 | -0.07 | 19,226 | 17,409 | -0.10 | 14,425 | 14,079 | -0.02 |
hsa-miR-19b | 13,365 | 9,406 | -0.42 | 25,463 | 22,438 | -0.13 | 21,532 | 20,625 | -0.04 | 18,039 | 17,440 | -0.03 |
hsa-miR-30e-5p | 9,497 | 6,838 | -0.39 | 13,045 | 11,321 | -0.15 | 12,643 | 10,887 | -0.16 | 13,230 | 11,809 | -0.12 |
hsa-let-7a | 15,244 | 4,335 | -2.52. | 28,304 | 6,181 | -3.58 *** | 22,226 | 6,386 | -2.48*** | 30,449 | 5,612 | -4.43*** |
hsa-let-7b | 8,236 | 539 | -14.28*** | 12,563 | 475 | -25.44*** | 12,150 | 500 | -23.30 *** | 12,309 | 452 | -26.24*** |
hsa-miR-203 | 907 | 325 | -1.79** | 1,155 | 348 | 2.32*** | 1,302 | 359 | -2.63*** | 1,186 | 5,757 | -1.07** |
hsa-miR-130b | 5,700 | 8,316 | -0.46 | 13,023 | 13,880 | 0.07 | 9,994 | 14,055 | 0.41 | 12,053 | 12,432 | 0.03 |
hsa-miR-455 | 2,677 | 2,604 | -0.03 | 3,944 | 5,285 | 0.34* | 3,437 | 5,927 | 0.72** | 3,262 | 5,342 | 0.64* |
b | ME-15 | HuH7 | ||||||||||
4 h | 24 h | 4 h | 24 h | |||||||||
-IFNa | +IFNa | CHF | -IFNa | +IFNa | CHF | -IFNa | +IFNa | CHF | -IFNa | +IFNa | CHF | |
Interferon-regulated microRNAs | ||||||||||||
hsa-miR-33b | 1,226 | 3,484 | 1.84** | 2,888 | 2,318 | -0.25 | 476 | 1,113 | 1.34** | 1,306 | 1,851 | 0.42*** |
hsa-miR-33 | 2,631 | 7,352 | 1.79 | 9,774 | 5,764 | -0.70* | 1,887 | 6,645 | 2.52. | 8,893 | 2,726 | -2.26 |
hsa-miR-126* | 2,221 | 5,409 | 1.44* | 5,578 | 6,111 | 0.10 | 4,749 | 6,410 | 0.35. | 5,687 | 5,803 | 0.02*** |
hsa-miR-10b | 1,695 | 3,564 | 1.10* | 2,929 | 4,297 | 0.47** | 378 | 411 | 0.09 | 371 | 382 | 0.03 |
hsa-miR-551b | 2,085 | 4,169 | 1.00* | 4,423 | 3,419 | -0.29 | 1,804 | 4,979 | 1.76* | 5,221 | 4,865 | -0.07 |
hsa-miR-137 | 1,037 | 1,966 | 0.90 | 2,523 | 2,872 | 0.14 | 1,613 | 3,155 | 0.96** | 3,429 | 3,598 | 0.05 |
hsa-miR-138 | 2,158 | 4,074 | 0.89* | 4,709 | 3,701 | -0.27 | 725 | 828 | 0.14 | 903 | 859 | -0.05 |
hsa-miR-130b | 5,700 | 9,994 | 0.75 | 13,023 | 12,053 | -0.08 | 8,316 | 14,055 | 0.69** | 13,880 | 12,432 | -0.12 |
hsa-miR-101 | 6,701 | 11,387 | 0.70 | 13,088 | 11,688 | -0.12 | 3,569 | 10,871 | 2.05** | 10,445 | 10,796 | 0.03 |
hsa-miR-140 | 7,339 | 12,236 | 0.67 | 15,512 | 15,931 | 0.03 | 3,058 | 5,976 | 0.95* | 6,135 | 7,221 | 0.18 |
HS_92 | 829 | 1,356 | 0.64. | 1,246 | 1,179 | -0.06 | 407 | 587 | 0.44 | 492 | 478 | -0.03 |
hsa-miR-362 | 1,382 | 2,234 | 0.62* | 2,144 | 2,276 | 0.06 | 1,907 | 2,737 | 0.43* | 2,456 | 2,519 | 0.03 |
hsa-miR-19b | 13,365 | 21,532 | 0.61** | 25,463 | 18,039 | -0.41 | 9,406 | 20,625 | 1.19* | 22,438 | 17,440 | -0.29 |
hsa-miR-130a | 13,031 | 20,878 | 0.60. | 27,571 | 23,048 | -0.20 | 17,884 | 31,012 | 0.73* | 33,801 | 28,875 | -0.17 |
hsa-miR-579 | 552 | 816 | 0.48. | 992 | 1,053 | 0.06 | 729 | 1,362 | 0.87* | 1,250 | 1,308 | 0.05 |
hsa-miR-29b | 23,138 | 34,148 | 0.48. | 32,691 | 29,710 | -0.10 | 8,737 | 17,989 | 1.06* | 20,599 | 17,405 | -0.18. |
hsa-miR-19a | 13,617 | 19,226 | 0.41* | 18,318 | 14,425 | -0.27 | 6,726 | 17,409 | 1.59. | 17,178 | 14,079 | -0.22. |
hsa-miR-338 | 1,298 | 1,813 | 0.40. | 1,870 | 1,603 | -0.17 | 2,363 | 4,603 | 0.95. | 5,109 | 4,088 | -0.25 |
hsa-miR-590 | 1,403 | 1,949 | 0.39. | 1,545 | 1,367 | -0.13 | 669 | 1,068 | 0.60. | 884 | 847 | -0.04 |
hsa-miR-545 | 1,455 | 1,973 | 0.36. | 2,149 | 1,720 | -0.25* | 829 | 1,371 | 0.65** | 1,573 | 1,393 | -0.13 |
hsa-miR-30e-5p | 9,497 | 12,643 | 0.33 | 13,045 | 132,030 | 0.01 | 6,838 | 10,887 | 0.59* | 11,321 | 11,809 | 0.04 |
hsa-miR-570 | 1,989 | 2,576 | 0.30. | 2,397 | 2,610 | 0.09 | 2,213 | 3,739 | 0.69* | 4,312 | 3,708 | -0.16 |
hsa-miR-301 | 13,621 | 17,531 | 0.29* | 16,321 | 15,142 | -0.08 | 5,646 | 10,460 | 0.85* | 10,925 | 10,295 | -0.06. |
hsa-miR-561 | 517 | 621 | 0.20 | 568 | 464 | -0.22** | 683 | 1,157 | 0.69** | 1,149 | 941 | -0.22 |
HS_250 | 4,284 | 1,813 | -1.36. | 740 | 983 | 0.33 | 3,688 | 2,337 | -0.58 | 1,195 | 1,303 | 0.09 |
c | ME-15 | HuH7 | ||||||||||
4 h | 24 h | 4 h | 24 h | |||||||||
-IFNa | +IFNa | CHF | -IFNa | +IFNa | CHF | -IFNa | +IFNa | CHF | -IFNa | +IFNa | CHF | |
Validated microRNAs | ||||||||||||
hsa-miR-19a | 13,617 | 19,226 | 0.41* | 18,318 | 14,425 | -0.27 | 6,726 | 17,409 | 1.59. | 17,178 | 14,079 | -0.22 |
hsa-miR-19b | 13,365 | 21,532 | 0.61** | 25,463 | 18,039 | -0.41 | 9,406 | 20,625 | 1.19* | 22,438 | 17,440 | -0.29. |
hsa-miR-30e-5p | 9,497 | 12,643 | 0.33 | 13,045 | 13,230 | 0.01 | 6,838 | 10,887 | 0.59* | 11,321 | 11,809 | 0.04 |
hsa-let-7a | 15,244 | 22,226 | 0.46 | 28,304 | 30,449 | 0.08 | 4,335 | 6,386 | 0.47. | 6,181 | 5,612 | -0.10 |
hsa-let-7b | 8,236 | 12,150 | 0.48* | 12,563 | 2,309 | -0.02 | 539 | 500 | -0.08 | 475 | 452 | -0.05 |
hsa-miR-203 | 907 | 1,302 | 0.44* | 1,155 | 1,186 | 0.03 | 325 | 359 | 0.10 | 348 | 575 | 0.665** |