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Table 2 Modulation of microRNA expression by IFNα—4 and 24 h after stimulation

From: MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

 

Control

Interferon-alpha treated

 

4 h

24 h

4 h

24 h

a

ME-15

HuH7

CHF

ME-15

HuH7

CHF

ME-15

HuH7

CHF

ME-15

HuH7

CHF

Validated microRNAs

hsa-miR-19a

13,617

6,726

-1.02

18,318

17,178

-0.07

19,226

17,409

-0.10

14,425

14,079

-0.02

hsa-miR-19b

13,365

9,406

-0.42

25,463

22,438

-0.13

21,532

20,625

-0.04

18,039

17,440

-0.03

hsa-miR-30e-5p

9,497

6,838

-0.39

13,045

11,321

-0.15

12,643

10,887

-0.16

13,230

11,809

-0.12

hsa-let-7a

15,244

4,335

-2.52.

28,304

6,181

-3.58 ***

22,226

6,386

-2.48***

30,449

5,612

-4.43***

hsa-let-7b

8,236

539

-14.28***

12,563

475

-25.44***

12,150

500

-23.30 ***

12,309

452

-26.24***

hsa-miR-203

907

325

-1.79**

1,155

348

2.32***

1,302

359

-2.63***

1,186

5,757

-1.07**

hsa-miR-130b

5,700

8,316

-0.46

13,023

13,880

0.07

9,994

14,055

0.41

12,053

12,432

0.03

hsa-miR-455

2,677

2,604

-0.03

3,944

5,285

0.34*

3,437

5,927

0.72**

3,262

5,342

0.64*

b

ME-15

HuH7

 

4 h

24 h

4 h

24 h

 

-IFNa

+IFNa

CHF

-IFNa

+IFNa

CHF

-IFNa

+IFNa

CHF

-IFNa

+IFNa

CHF

Interferon-regulated microRNAs

hsa-miR-33b

1,226

3,484

1.84**

2,888

2,318

-0.25

476

1,113

1.34**

1,306

1,851

0.42***

hsa-miR-33

2,631

7,352

1.79

9,774

5,764

-0.70*

1,887

6,645

2.52.

8,893

2,726

-2.26

hsa-miR-126*

2,221

5,409

1.44*

5,578

6,111

0.10

4,749

6,410

0.35.

5,687

5,803

0.02***

hsa-miR-10b

1,695

3,564

1.10*

2,929

4,297

0.47**

378

411

0.09

371

382

0.03

hsa-miR-551b

2,085

4,169

1.00*

4,423

3,419

-0.29

1,804

4,979

1.76*

5,221

4,865

-0.07

hsa-miR-137

1,037

1,966

0.90

2,523

2,872

0.14

1,613

3,155

0.96**

3,429

3,598

0.05

hsa-miR-138

2,158

4,074

0.89*

4,709

3,701

-0.27

725

828

0.14

903

859

-0.05

hsa-miR-130b

5,700

9,994

0.75

13,023

12,053

-0.08

8,316

14,055

0.69**

13,880

12,432

-0.12

hsa-miR-101

6,701

11,387

0.70

13,088

11,688

-0.12

3,569

10,871

2.05**

10,445

10,796

0.03

hsa-miR-140

7,339

12,236

0.67

15,512

15,931

0.03

3,058

5,976

0.95*

6,135

7,221

0.18

HS_92

829

1,356

0.64.

1,246

1,179

-0.06

407

587

0.44

492

478

-0.03

hsa-miR-362

1,382

2,234

0.62*

2,144

2,276

0.06

1,907

2,737

0.43*

2,456

2,519

0.03

hsa-miR-19b

13,365

21,532

0.61**

25,463

18,039

-0.41

9,406

20,625

1.19*

22,438

17,440

-0.29

hsa-miR-130a

13,031

20,878

0.60.

27,571

23,048

-0.20

17,884

31,012

0.73*

33,801

28,875

-0.17

hsa-miR-579

552

816

0.48.

992

1,053

0.06

729

1,362

0.87*

1,250

1,308

0.05

hsa-miR-29b

23,138

34,148

0.48.

32,691

29,710

-0.10

8,737

17,989

1.06*

20,599

17,405

-0.18.

hsa-miR-19a

13,617

19,226

0.41*

18,318

14,425

-0.27

6,726

17,409

1.59.

17,178

14,079

-0.22.

hsa-miR-338

1,298

1,813

0.40.

1,870

1,603

-0.17

2,363

4,603

0.95.

5,109

4,088

-0.25

hsa-miR-590

1,403

1,949

0.39.

1,545

1,367

-0.13

669

1,068

0.60.

884

847

-0.04

hsa-miR-545

1,455

1,973

0.36.

2,149

1,720

-0.25*

829

1,371

0.65**

1,573

1,393

-0.13

hsa-miR-30e-5p

9,497

12,643

0.33

13,045

132,030

0.01

6,838

10,887

0.59*

11,321

11,809

0.04

hsa-miR-570

1,989

2,576

0.30.

2,397

2,610

0.09

2,213

3,739

0.69*

4,312

3,708

-0.16

hsa-miR-301

13,621

17,531

0.29*

16,321

15,142

-0.08

5,646

10,460

0.85*

10,925

10,295

-0.06.

hsa-miR-561

517

621

0.20

568

464

-0.22**

683

1,157

0.69**

1,149

941

-0.22

HS_250

4,284

1,813

-1.36.

740

983

0.33

3,688

2,337

-0.58

1,195

1,303

0.09

c

ME-15

HuH7

 

4 h

24 h

4 h

24 h

 

-IFNa

+IFNa

CHF

-IFNa

+IFNa

CHF

-IFNa

+IFNa

CHF

-IFNa

+IFNa

CHF

Validated microRNAs

hsa-miR-19a

13,617

19,226

0.41*

18,318

14,425

-0.27

6,726

17,409

1.59.

17,178

14,079

-0.22

hsa-miR-19b

13,365

21,532

0.61**

25,463

18,039

-0.41

9,406

20,625

1.19*

22,438

17,440

-0.29.

hsa-miR-30e-5p

9,497

12,643

0.33

13,045

13,230

0.01

6,838

10,887

0.59*

11,321

11,809

0.04

hsa-let-7a

15,244

22,226

0.46

28,304

30,449

0.08

4,335

6,386

0.47.

6,181

5,612

-0.10

hsa-let-7b

8,236

12,150

0.48*

12,563

2,309

-0.02

539

500

-0.08

475

452

-0.05

hsa-miR-203

907

1,302

0.44*

1,155

1,186

0.03

325

359

0.10

348

575

0.665**

  1. Microarray data for RT-PCR validated microRNAs, see description of Table 1. Average relative fluorescent levels and their change factors (CHFs) are shown for hepatoma (HuH7) and melanoma (ME-15) cells at two time points. Genes were selected by significance calculated in a linear model with interaction assuming that the change may not occur in both cell lines (e.g. miR-10b has no detectable levels in HuH7). p value significance codes are defined by the intervals: '***' < 0.001 ≤ '**' < 0.01 ≤ '*' < 0.05 ≤ '.' < 0.1. Section c shows the microarray expression levels of microRNAs assayed by RT-PCR (treatment analysis)