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Figure 3 | Biological Procedures Online

Figure 3

From: MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

Figure 3

IFNα-dependent modulation of microRNA expression. a Volcano plot display of IFNα induced microRNA upregulation 4 h after induction. The change factor values (2^log factor change -1) are plotted on the X-axis against the p value in logarithmic scale on the Y-axis. Top-rated microRNAs are annotated together with the let-7 family members. b Confirmation of IFNα effect for selected microarray data by qPCR. The CT-values are the average of three technical and three biological replicates and changes were calculated with 2^ΔMNE (mean normalized expression values). Error bars show 2^ΔMNE ± Δx (average standard error of treated and untreated MNE) from biological triplicates. miR-30e failed to amplify in ME-15 and miR-203 was below the detection limit in HuH7. Expression values were normalized against endogenous snoRNA RNU48 levels.

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